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Gopinath, C.
- Development and Validation of UV-Spectrophotometric Method for Determination of Cephalexin
Abstract Views :574 |
PDF Views:2
Authors
G. Venkata Prasad
1,
S. Sravani
1,
B. Mohammed Ishaq
1,
M. Madhu
1,
Sreenivasulu Munna
1,
C. Gopinath
1
Affiliations
1 Department of Pharmaceutical Analysis, Annamacharya college of Pharmacy, Rajampet, Kadapa Dist, A.P, IN
1 Department of Pharmaceutical Analysis, Annamacharya college of Pharmacy, Rajampet, Kadapa Dist, A.P, IN
Source
Asian Journal of Research in Chemistry, Vol 6, No 5 (2013), Pagination: 490-494Abstract
To develop simple, sensitive, selective and accurate UV Spectrophotometric methods for the determination of Cephalexin in bulk drug and pharmaceutical formulations (Capsules). The absorbances were measured at 261(Method A) and 257 nm (Method B). The methods was found to be linear from a quantitation ranges of 5μg/ml to 40μg/ml (Method A) in Phosphate Buffer pH 2.0 and 5μg/ml to 50μg/ml (Method B) in 0.1 N HCl. The regression of the curves was Y = 0.020x - 0.021 (Method A) and Y = 0.014x - 0.102 (Method B). The methods gave satisfactory results in terms of repeatability and intermediate precision (RSD<1.010%) (Method A) and (RSD<0.589%) (Method B) 0.855 μg/mL and 2.85 μg/mL (Method A) and 2.357 μg/mL and 7.857 μg/mL were the LOD and LOQ values, respectively. The methods was validated and proved to be robust and rugged. The results of analysis for these methods have been validated statistically and by recovery studies.Keywords
Cephalexin, Spectroscopic Method, Phosphate Buffer (Ph 2.0), 0.1 N HclReferences
- The United States Pharmacopoeia XXIII. 1993. United States Pharmacopoeia Convection. Rockville, Maryland, U. S. A.
- Agbaba D. et al., HPTLC assay of cephalexin and cefaclor in pharmaceuticals. Biomedical Chromatography, 1998, 12, 133- 135.
- Halkar U.P. and Bhandari N.P. Simultaneous determination of cephalexin and probenecid in pharmaceutical preparations by HPTLC. Indian Drugs, 1998, 35, 382–383.
- Coran S.A. et. al., Development of a densitometry method for the determination of cephalexin as an alternative to the standard HPLC procedure. Journal of Pharmaceutical and Biomedical Analysis., 1998, 18, 271–274.
- Bhooshan R. and Prashad V., Separation and Identification of Some Cephalosporin’s on Impregnated TLC Plates., Biomedical Chromatography, 1996, 10, 258–260.
- Najib N. M. et. al., High performance liquid chromatographic analysis of cephalexin in serum and urine., Journal of Clinial pharmacy and therapeutics, 2008, 12, 419-426.
- Zendelovska D. et. Al., Simultaneous quantification of cefaclor and cephalexine in bloodplasma using high-performance liquid chromatography with UV detection., Acta Pharm,2002, 52, 243– 250.
- Lee Y.J. and Lee H.S., Simultaneous determination of cefoxitin, cefuroxime, cephalexin and cephaloridine in plasma using HPLC and a column-switching technique. Chromatographia, 1990, 30, 80–84.
- Prayanka P. and Suresh P., Development of colorimetric method for cephalexin in dosage forms, Asian Journal of Pharmaceutics, 2008, 2, 120–122.
- Patel S.A. et. al., Spectrophotometric methods for the estimation of Cephalexin in tablet dosage forms., Indian Journal of Pharmaceutical Sciences, 2006, 68, 278-280.
- Validation of Analytical Procedures: Text And Methodology Q1A (R2), ICH Harmonized Tripartite Guideline, Feb 2003.
- Green J M, A practical guide to analytical method validation, Analytical Chemistry: 1996, 305A.
- Synthesis, Characterization, Docking and Biological Evaluation of Tetra Hydro Imidazo [1, 2-A] Pyrazine Derivatives
Abstract Views :188 |
PDF Views:2
Authors
Affiliations
1 Dept. of Pharmaceutical Chemistry, QIS College of Pharmacy, Vengamukka Palem, Ongole, 523272, IN
2 Dept. of Pharmaceutical Chemistry, Annamacharya College of Pharmacy, Rajampet, 516126, IN
3 Dept. of Pharmacognosy, Annamacharya College of Pharmacy, Rajampet, 516126, IN
1 Dept. of Pharmaceutical Chemistry, QIS College of Pharmacy, Vengamukka Palem, Ongole, 523272, IN
2 Dept. of Pharmaceutical Chemistry, Annamacharya College of Pharmacy, Rajampet, 516126, IN
3 Dept. of Pharmacognosy, Annamacharya College of Pharmacy, Rajampet, 516126, IN
Source
Asian Journal of Research in Chemistry, Vol 9, No 6 (2016), Pagination: 271-276Abstract
Novel Tetra hydro imidazo[1,2-a] pyrazine derivatives have been synthesized by Treatment of pyrazine-2- carboxylic acid with thionyl chloride in methanol yielded methyl pyrazine-2-carboxylate.This up on treatment with alcoholic ammonia gave pyrazinamide, further it was undergone Hoffman's degradation, gave 2-amino pyrazine after that it was refluxed for 26 hrs with chloro acetaldehyde , sodium bicarbonate gave imidazo[1,2-a] pyrazine and hydrogen gas was passed on it in the presence of palladium on carbon yielded tetra hydro imidazo[1,2-a] pyrazine ,which was treated with different substituent's to get the novel derivatives. Synthesized compounds were identified and characterized by Melting point, TLC, FT-IR, 1H and 13C NMR , MASS and percent purity done by HPLC . Based on characterization and percent purity novel derivatives were subjected for molecular docking , QSAR studies and pharmacological evaluation. Selected compounds were docked on Antioxidant activity using "Thioredoxin peroxidase B" as a target site. QSAR parameters like partition coefficient was studied and showed polar surface area below 140 A0 and passed ADME parameters. Analysis of docking and QSAR studies suggested that remarkable inhibitory activity was exhibited by the derivatives. Hydrogen bond interactions were mapped to confirm their potencies. They were evaluated for In-Vitro Anti-oxidant activity by DPPH method and In-Vitro Anti-diabetic activity by alpha-amylase inhibition assay by DNSA method. Results suggested that electron withdrawing groups like nitro, bromo having compounds shown potent activity. Rest of compounds showed mild to moderate activity.Keywords
Tetra Hydro Imidazo[1,2-A] Pyrazine, Docking and Q SAR, Anti-Oxidant, Anti-Diabetic Activities.- Synthesis, Characterization of 3-chloro 4-(4- Substituted Phenyl -1- (4- Nitro Phenylazetidin -2-one by Microwave Method and Evaluation of their Antibacterial Activity
Abstract Views :205 |
PDF Views:2
Authors
Affiliations
1 Dept. of Pharmaceutical Chemistry, Annamacharya College of Pharmacy, New Boyanapalli, Rajampet, Kadapa (Dist) A.P, 516115, IN
2 Dept. of Pharmaceutical Chemistry, Annamacharya College of Pharmacy, New Boyanapalli, Rajampet, Kadapa (Dist) A.P, 516115, IN
1 Dept. of Pharmaceutical Chemistry, Annamacharya College of Pharmacy, New Boyanapalli, Rajampet, Kadapa (Dist) A.P, 516115, IN
2 Dept. of Pharmaceutical Chemistry, Annamacharya College of Pharmacy, New Boyanapalli, Rajampet, Kadapa (Dist) A.P, 516115, IN